Function Point Analysis Template

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RGS12 is required for the upkeep of mitochondrial feature all through skeletal development Cartilage-specific RGS12-knockout (KO) mice To generate cartilage-certain RGS12 KO mice, RGS12fl/fl mice had been crossed with mice expressing cre recombinase below the control of the Col2-promotor (Col2-cre+). Col2-cre+, and the KO mice were littermates derived from the breeding of heterozygous animals. The animals have been maintained beneath particular pathogen-free conditions. All animal stories have been performed in response to institutional guidelines and with approval by using the Institutional Animal Care and Use Committee (IACUC) of tuition of Pennsylvania. boom curves For phenotypic characterization, the weight of cartilage-certain RGS12 KO and manage littermates turned into determined at postnatal days 1, 7, 14, 21, 28, and fifty six of age. mobile tradition and transfection Cartilage of the knee and hip joints changed into harvested from 2-day-historic mice followed by using digestion with 0.2% collagenase (Thermo Scientific, US) for 4 h. basic chondrocytes have been grown in DMEM supplemented with 10% FBS and antibiotics that were modified every 3 days except the cells had been confluent. fundamental chondrocytes have been seeded on 6-smartly plates at three × one hundred and five cells/smartly (~90–ninety five% confluency). Cells had been transfected on here day with FuGENE HD Transfection Reagent (E2311, Promega enterprise, US). Cells had been harvested at 48 h after transfection. Mitochondrial isolation Mitochondria had been isolated from cartilage using a mitochondrial isolation kit (Novus Biologicals, US) based on the brand’s directions. After washing with PBS, cartilage tissues have been suspended and homogenized with extraction buffer and then centrifuged at 600 × g for 10 min at four °C. The supernatant changed into moreover centrifuged at 11,000 × g for 10 min to compile the final pellet, which consisted of mitochondrial protein. The concentration of mitochondrial protein changed into measured using a BCA Protein Assay kit (Thermo Fisher Scientific, US). Plasmid development the entire size of the RGS12 cDNA fragment (NM_173402.2) turned into cloned and inserted into the p3xFLAG-Myc-CMV-26 spine. Full-length ATP5A cDNA (often known as Atp5f1a, NM_007505) was cloned and inserted into the pCMV6-Myc spine. shRNA construction and virus production Nontargeting RGS12 shRNAs had been received from GeneCopoeia, Inc. goal sequences have been as follows: manage shRNA: GCTTCGCGCCGTAGTCTTA; RGS12 shRNA1: CTAGGCAAGTCTAACTCTATT; RGS12 shRNA2: CCTGTCCATGATTAATAAAGG; and RGS12 shRNA3: AGTCTGCAACTGTGTCTGATGGCGAGTTG. Nontargeting ATP5A shRNAs had been obtained from Santa Cruz Biotechnology (sc-60228-SH, Dallas, TX). The 293 T packaging phone line turned into used for lentiviral amplification48. in short, viruses have been gathered at 72 h post-transfection. After passing via 0.forty five-µm filters, viruses had been used to infect chondrocytes. Western blot Cartilage or chondrocytes have been homogenized with RIPA (radioimmunoprecipitation assay) buffer containing PIC (Protease Inhibitor Cocktails) (Sigma–Aldrich, US) on ice. Protein concentration became measured the usage of BCA protein assay reagent (Thermo Fisher Scientific). Equal amounts of protein (30 μg) have been denatured in SDS and separated in 10% SDS-page gels. Proteins have been transferred to PVDF membranes in transfer buffer containing 20% methanol. The membranes have been blocked with 5% skim milk, incubated with primary antibody overnight at four °C and then incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:2,000, Jackson ImmunoResearch, PA) at room temperature for 1 h. α-TUBLIN (1:2,000, 11224-1-AP, Proteintech), β-ACTIN (1:2000, sc-47778, Santa Cruz) or COXIV (1:5000, 11242-1-AP, Proteintech) turned into used as the interior control. here primary antibodies have been used: anti-RGS12 (1:1,000, GW21317, Sigma–Aldrich), anti-ATP5A (1:1,000, 14676-1-AP, Proteintech), complete OXPHOS Rodent WB Antibody Cocktail (1:a thousand, Ab110413, Abcam), anti-p-Tyr (1:one thousand, Ab10321, Abcam), anti-BAX (1:2000, 50599-2-Ig, Proteintech), anti-BCL-2 (1:2000, 12789-1-AP, Proteintech), anti-professional-CASPASE-eight (1:2000, 4790, cellphone Signaling know-how), anti-cleaved-CASPASE-8 (1:2000, 8592, mobilephone Signaling technology), anti-seasoned-CASPASE-9 (1:2000, 9504, mobile Signaling expertise), and anti-cleaved-CASPASE-9 (1:2000, 9509, cell Signaling expertise). Coimmunoprecipitation ADTC5 cells (CRL-2846, ATCC) were lysed in NP-40 buffer supplemented with PIC and phenylmethylsulfonyl fluoride (Sigma–Aldrich, US). in short, lysates of equal amounts of protein have been incubated at room temperature with fundamental antibodies for 1 h after which with protein A/G beads overnight, after which the beads were washed with PBST. bound proteins had been solubilized in loading buffer for western blot analysis. Histological evaluation Mouse knee joints have been exposed by using removing the overlying skin and have been consequently excised and decalcified with 0.5 M EDTA (pH = eight) for 14 days. A transverse reduce become made when the bones have been thoroughly decalcified and processed for paraffin embedding. Tissues have been cut into eight-µm sections and stained with hematoxylin/eosin or Safranin O/speedy green to check joint pathology. Immunofluorescence Coverslips had been incubated with basic antibodies towards ATP5A (1:1,000, 14676-1-AP, Proteintech), RGS12 (1:1,000, GW21317, Sigma–Aldrich), cleaved-CASPASE-three (1:2000, 9664, phone Signaling expertise), and MitoTracker (1:100, M7514/M7512, Thermo Fisher Scientific) overnight at 4 °C. Cells had been then incubated with the corresponding fluorochrome-conjugated secondary antibodies (diluted 1:500 in 1% BSA) for 1 h at room temperature. The relative fluorescence depth was determined by way of evaluating every intensity price to the average depth of cells. Reactive oxygen species The DCFDA (2′,7′-dichlorofluorescein diacetate) assay turned into used to analyze intracellular ROS degrees. Chondrocytes have been incubated with 10 μM DCFDA in serum-free medium at 37 °C for 30 min. subsequently, ROS levels have been immediately assessed using a fluorescence microscope (Leica, GER) to become aware of the mean fluorescence intensity (MFI) with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. Mitochondrial membrane expertise The mitochondrial membrane competencies (MMP) of chondrocytes changed into assessed the use of the fluorescent dye rhodamine 123. Chondrocytes were cultured in a 6-neatly plate and due to this fact stained with 2 µM rhodamine 123 for 30 min at 37 °C in the dark. Cells had been analyzed under an inverted fluorescence microscope. The fluorescence intensity changed into calculated with LAS application (V4.3) (Leica Microsystems GmbH, Wetzlar, Germany). ADP, ATP, and ADP/ATP ratio Intracellular ADP, ATP, and the ADP/ATP ratio have been measured by way of an ADP/ATP Ratio Assay kit (BioAssay programs, US). basic chondrocytes had been seeded in white opaque microplates and treated with ATP/ADP reagent (BioAssay systems, US). The ATP and ADP content material within the supernatants changed into quantified the use of a tremendously delicate luciferase-based mostly approach. Luciferase endeavor become measured on a luminometer (BioTek, US) and compared with necessities. Microcomputed tomography (micro-CT, μCT) Two-dimensional pictures of the tibia by using microcomputed tomography analysis were received. The parameters, bone mineral density (BMD; mg/cm), bone quantity fraction (BV/television; %), trabecular thickness (Tb.Th; mm), trabecular quantity (Tb.N; 1/mm), and trabecular separation (Tb.Sp; mm), were calculated the usage of micro-CT software (Bone J, NIH). Quantitative true-time qPCR evaluation RNA from mouse cartilage or chondrocytes changed into isolated the use of TRIzol reagent (Thermo Fisher Scientific, country) in accordance with the manufacturer’s guidance. Then, 1 µg of RNA become reverse transcribed into cDNA the use of the Reverse Transcription equipment (TAKARA, Japan). real-time PCR became performed with a response blend containing primers, the cDNA template, and 2x SYBR eco-friendly qPCR grasp mix (Bimake, country). The sequences of primers are proven in Supplementary desk S1. mtDNA copy quantity The relative mtDNA replica number become decided with the aid of polymerase chain reaction (PCR). complete DNA from chondrocytes became extracted the usage of a DNeasy Blood & Tissue package (QIAGEN, US) in line with the brand’s instructions. The mt-COX1 and mt-ND1 genes were amplified to check the relative mtDNA reproduction quantity, with the sixteen S gene used for normalization. The sequences of primers are proven in Supplementary desk S1. WST-1 viability assay primary chondrocytes were plated in triplicate in ninety six-smartly plates (one thousand cells/well) in appropriate growth medium. The cell proliferation/viability assay turned into carried out with the aid of following the WST-1 package guidelines (Sigma_Aldrich, usa). in brief, on the indicated time aspects, telephone medium became changed with ninety µL clean increase medium supplemented with 10 µL WST-1 reagents, adopted by incubation at 37 °C for 1 h. OD absorbance values were measured at 490 nm the use of a ninety six-smartly plate reader (Bio-Rad, u . s . a .). Transmission electron microscopy basic chondrocytes from C57BL/6 mice had been fixed overnight. Nanometer sections had been cut and stained with 0.3% (w/v) lead citrate, and images had been analyzed at the Electron Microscopy Core Facility. For Immunoelectron microscopy (IEM), fundamental chondrocytes were cultured on plastic coverslips and glued in 0.25% glutaraldehyde in PBS for 1 h at room temperature. After permeabilization in PBS containing 0.25% saponin for 30 min followed by using blocking off for 30 min in PBS containing 10% bovine serum albumin, the cells had been exposed in a single day to mouse simple antibodies within the blocking solution. The specimens were incubated with gold-conjugated goat anti-mouse-IgG (Abcam, 27241) in the blocking answer for 1 h at room temperature. The specimens had been postfixed in 1% OsO4 containing 1.5% potassium ferrocyanide and had been processed for electron microscopy in a similar way to the protocol for common electron microscopy. Statistical analysis All data are expressed because the suggest ± S.E.M. Statistical importance was decided by using unpaired two-tailed student’s t-examine. An analysis of variance verify turned into first performed to examine the imply values between companies, and the pupil–Newman–Keuls verify changed into used to examine the mean values between two situations with GraphPad application 7.0 (San Diego, CA, US). In all cases, P-values under 0.05 had been considered enormous. three sorts of advertising dashboards and how to make use of them marketing groups have loads of relocating items to retain tune of, with distinct channels, campaigns, audiences and key performance symptoms (KPIs) to computer screen. How can your usual marketer hope to dwell on excellent of all of it? That’s the place advertising and marketing dashboards come into play. These structures deliver precise-time analytics and insights into your advertising activities, so you can get a complete view of your marketing performance and admire the place changes deserve to be made. what’s a advertising dashboard? feel of a marketing dashboard as your command middle for monitoring the efficiency of your distinct advertising and marketing campaigns and methods. These platforms give a top level view of the KPIs and advertising metrics attached to a number of advertising activities. advertising dashboards offer visible representations of key advertising and marketing metrics and provides marketers — and more importantly, their managers — a short overview of the status of your advertising campaigns. advertising analytics and KPIs can cowl loads of ground, from tracking advertising spend throughout distinct areas to monitoring conversion quotes and revenue profits. source: Datorama in many cases, you’ll likely discover that you simply need a dedicated marketing dashboard for certain facets of your advertising approach including: search engine optimization (web optimization), social media, e-mail advertising, and many others. Generalized digital advertising dashboards offer a excessive-level view of your advertising and marketing efforts, which is useful in and of itself, however they may additionally no longer drill down as far into every pillar of your advertising crusade as a greater really expert solution will. The respectable information is many main advertising tools, like Google Analytics and Salesforce, help you build your personal advertising dashboards for different makes use of. You simply deserve to recognize a way to use the accessible customization alternate options to create the greatest dashboard for a specific software. Why are marketing dashboards important? every marketer feels the drive to bring outcomes, whether success measured in conversion costs, engagement metrics or decent ancient-common income salary. Analytics dashboards give marketing groups the insights they deserve to exhibit ROI to their key decision-makers. need to demonstrate enterprise leaders your advertising efforts are paying off? The visual charts and diagrams included in these structures are a good method to blow their own horns your advertising wins. however let’s say the prognosis isn’t so first rate, and your marketing efforts are failing to hit your quarterly desires. developed-in analytics tools permit you to improved take into account why your techniques aren’t performing at a high stage and where they’re failing to connect with your target viewers and customer base. It’s inconceivable to right the ship if you don’t recognize why you’ve gone off course within the first vicinity. advertising dashboards deliver the insights and analysis to point you back within the right direction. real-time analytics are vital, offering marketing groups with performance updates always. you can reply dynamically to shifting audience, market or trade trends to continuously hold your advertising and marketing suggestions on element. All in all, these dashboards are a good technique to condense your advertising and marketing analytics and information into a visually attractive format that’s easily digestible for advertising and marketing executives and different essential stakeholders and decision-makers. What forms of marketing dashboards should still be used? As we noted, there are tons of distinct marketing dashboards to choose between, and a lot of of these systems focus on certain add-ons to your advertising method. whatever classification of campaign you’re working, there’s a advertising dashboard accessible so as to provide you with greater perception into these advertising and marketing activities. listed below are one of the most essential alternate options to believe: 1. search engine optimisation advertising and marketing dashboard every marketer wishes to look their site rating on the excellent of search engine effects pages (engines like google) for their most extremely coveted keyword phrases. And with the precise 3 positions accounting for seventy five% of all Google search clicks, it’s more critical than ever to take expertise of search engine optimisation tools to move your method up those search engines like google and seize that precious precise property. SEO is a sophisticated mixture of on-page, off-page and technical factors that directly affect your web site’s search ranking — and, by using extension, your capacity to entice greater organic site visitors. It’s a lot of ground to cowl, and sometimes the KPIs you deserve to tune can latitude from primary web page traffic to competitor key phrase search performance. each advertising crew needs a committed dashboard to computer screen their search engine optimization campaign performance so stakeholders can abruptly modify innovations that aren’t working and normally refine and enrich their advertising and marketing efforts. What platform if you happen to use on your SEO advertising performance dashboard wants? Google Analytics is an obvious option: It’s free. It’s customizable. It’s easy to integrate with other platforms. It’s consumer-friendly. It’s supported with the aid of Google itself. It’s the trade regular. in fact, it would be odd if a digital advertising group wasn’t the use of Google Analytics in some ability to measure and more suitable take into account web page performance. Of direction, even if you’re already the usage of Google Analytics, there are all the time the way to get greater out of the platform. There are a number of advertising teams that use Google Analytics to capture search engine optimization and site performance from a excessive degree, but under no circumstances in fact dig deeper into greater granular metrics and developments. Take knowledge of the platform’s customized reporting capabilities to song excessive-cost business touchdown pages, display screen selected advert or electronic mail campaigns, spoil down website conversions with the aid of audience classification and lots extra. the use of the collection of developed-in widgets and dashboard templates, you could tailor your analytics dashboard to zero in on the marketing KPIs that matter most to you. This illustration, courtesy of Moz, highlights a custom dashboard constructed to tune conversions from month to month. It’s easy for advertising executives to spot essential tendencies like spikes in conversion costs while not having to buckle down and do a bunch of statistical information. source: Moz 2. e mail marketing dashboard email is among the most crucial advertising channels, providing an ordinary return on investment of $42 for every $1 spent. to see that sort of ROI — or, ideally, surpass trade averages — groups deserve to intently monitor each point of their e mail advertising campaigns and make adjustments each time vital. Even the slightest misstep — a universal field line, as an example — can shrink back an email publication subscriber and lose a possible income lead, so manufacturers deserve to carefully analyze their email advertising campaigns to have in mind which recommendations are working. important e mail marketing metrics to track consist of: Open price. click-via cost. Conversion rate. leap expense. Unsubscribe price. electronic mail sharing or forwarding rate. e mail listing increase rate. general ROI. That’s simply the tip of the iceberg. It’s also a good idea to phase cellular metrics from other formats to account for consumer event and interface factors that could affect e-mail advertising and marketing efficiency. an excellent email advertising dashboard brings all of that facts together and applications it in a means that’s easy to understand and digest. extra importantly, it’s going to enable advertising and marketing teams to slice and dice records in order to focal point on the advertising and marketing KPIs that be counted most to them. as an instance, in case you’re working an e mail crusade as part of a broader content material advertising and marketing method, you might tune which sorts of content resonate probably the most together with your audience. Google Analytics has numerous widgets, marketing dashboard templates and customization equipment to support you drill into specific efficiency metrics. right here’s an illustration, showing the number of periods produced by using distinctive touchdown pages and items of content material connected in email newsletters: source: campaign display screen With that variety of versatility, Google Analytics can characteristic as both an electronic mail advertising and marketing and content marketing dashboard, together with its many different purposes. 3. Social media advertising and marketing dashboard preserving a powerful social media presence is crucial for B2B and B2C agencies alike. if you can’t see how your brand’s performing across different social media platforms, you may additionally no longer be constructing the stage of engagement you suppose you are. Given how straight away issues flow in the social media world, brands deserve to stay on excellent of their social media analytics 24/7. A committed social media advertising and marketing dashboard can support video display traits and traits in actual time and supply actionable insights that will also be immediately applied to your method. This intelligence can empower you to be extra responsive, swap your social media approach on the fly and hold evolving together with your goal audience. Social media advertising dashboards provide the tips mandatory to make it all occur. advertising and marketing teams can find the entire crucial social media advertising and marketing metrics they’re looking for in one handy to be aware interface. that you would be able to music key performance warning signs like: Followers. Likes. reach. Impressions. typical engagement rate. viewers growth rate. desirable rating posts. Social share of voice. There are a lot of decent options to make a choice from — as soon as once more, Google Analytics has equipment to help visualize social media analytics — however one of the crucial most suitable dedicated systems available is Sprout Social. that you can break down your social media advertising metrics by channel and phase different KPIs to trap a better view of your company’s efficiency. supply: Sprout Social With so many customization alternate options to explore, entrepreneurs can computer screen their social media presence from any vantage aspect they like, whether it’s a excessive-stage overview or an in-depth evaluation of a specific segment of your audience and followers. data must book every marketing choice you make, however for any person who doesn’t have a degree in records, wading via all of that assistance to find actionable insights can look insurmountable. Digital advertising and marketing dashboards use intuitive visual aids to simplify and analytics displays that carry probably the most crucial and useful advertising and marketing facts to the surface. if you can make essentially the most out of these systems, you’ll by no means need to motel to guesswork when managing your advertising campaigns. Ann Doherty: it’s time for flood defences in Cork metropolis In contemporary weeks, Cork’s Prince’s road has rightly been offered as an exemplar of how company can imaginatively function in the course of the Covid-19 pandemic. The collaboration of eating places, bars, agents, and Cork city Council created an open and shiny trading street, a template taken up by using many streets within the city and envied with the aid of different cities and cities. but have been it no longer for a change of wind route from southeasterly to southerly on the afternoon of Thursday, August 20, Prince’s street would have been badly flooded and its corporations broken once more – some doubtless irretrievably – and the development accomplished in contemporary months set to naught. The same fate also awaited many other streets within the metropolis centre including Oliver Plunkett road, Pembroke street, and cook street. The stakes at play in the extra obstruction of the Morrison’s Island public realm and flood defence scheme can not be laid out greater starkly than via these movements of August 20. On that day, morning forecasts indicated a possibility of flooding on a par with that skilled in February 2014. corporations, hanging on through a thread during the resilience and backbone of owners and team of workers, are now confronted by the prospect of legal challenges to the challenge. These agencies will proceed to operate at severe chance, uninsurable and unsure, while objectors stay away from a solution to flooding. That solution, the Morrisons Island scheme, changed into the subject of a full public consultation, assessment and amendment. Cork city Council appreciates the should take heed to the public, fully respects the judicial process and accepts the rights of people who object and problem tasks to do so. there is public consultation on all proposed infrastructural tasks within the metropolis, and differing views are regarded. Inevitably, even after public consultation, now not every thing accepted will discover frequent contract and some can be disappointed. however most likely people that want essential infrastructural tasks to proceed expeditiously even have rights; the appropriate to very own security, the appropriate to reside in the city centre and to perform enterprise devoid of the ever existing threat of flooding, the right to business continuity? Stripped down, the save Cork city (SCC) crusade’s narrative, not just in the case of the Morrison’s Island assignment however also to the reduce Lee Flood reduction Scheme (LLFRS) relies on a single ‘article of faith’: a philistine metropolis council that has no regard for heritage, history or way of life is in charge. My response to that’s with ease: “Please appear round you”. The city council has led on initiatives reminiscent of St Peter’s on North main road, Fenns Quay, Blackrock fortress Observatory, historical Cork Waterworks, Elizabeth citadel, and has facilitated or partnered on the refurbishment of Boole condominium, Frank O’Connor residence, Civic have faith condominium, Washington street Courthouse and Nano Nagle region. The Morrison’s Island scheme will end the use of the riverside as a giant motor vehicle park. it is going to create a three-metre large quayside promenade and enable people to enjoy the area. It gives a chance for broader regeneration. SCC presents itself as having a special perception of flood defence and climate trade beyond these State agencies and overseas consultants charged with and accountable for the beginning of defence schemes. site visitors makes its method through flooding on Lapp’s Quay, Cork. picture: Michael Mac Sweeney/Provision This has led them to posit a solution to the issue of flooding in Cork city centre and the wider Lee catchment – a tidal barrier at Little Island. SCC’s personal consultants, HR Wallingford, opposite to the impression created, did not design the barrier or recommend its region at Little Island, or supply, as has been claimed, “can charge walk in the park” for any such barrier. truly, before HR Wallingford demanded that SCC cease misrepresenting its report, they recommended that special hydrodynamic, navigational protection and environmental evaluation be carried out to come back to an counseled view on the tidal barrier. The only specific pre-feasibility study on the be counted become conducted by LLFRS and its 200-page look at features out that a tidal barrier at Little Island is not workable on the grounds of navigational safety, environmental impacts, local weather trade adaptability, and cost. while a correctly sited and designed tidal barrier, if or when required, may get to the bottom of the tidal difficulty, it will no longer handle the river flooding issue within the metropolis. nonetheless, this thought of a “silver bullet” within the kind of a tidal barrier persists and continues to be promulgated by using SCC. It seems that SCC wants to set apart a quantum of work undertaken by teams of international specialists over 13-years, which has been peer reviewed and internationally validated, in favour of a tidal barrier proposition that fails on all serious technical criteria. it’s additionally value noting every substantial challenge raised in submissions acquired all through the general public session section of LLFRS become addressed in the scheme exhibition document (together with appended reviews), which become made publicly available in December 2017. SCC will object to anything else that doesn’t meet its preferences. it’s going to lengthen and litigate towards all such tasks, regardless of public consultation, Bord Pleanála choices, international peer assessment, the commonplace decent. Do people who object to the Morrison’s Island Scheme have a “correct” to achieve this and by way of so doing, prolong the anxiousness of those that live in and have invested in the metropolis and who rely for their livelihoods there? completely. however is it good value, fair and proportionate? No, it isn’t. The Docklands to city Centre road network Scheme is also in SCC’s go hairs. Proposed with the aid of Cork city Council to give a gateway to the docklands and to provide drastically more desirable facilities for bus passengers, cyclists and pedestrians in addition to introducing new facilities to the enviornment, it’s a high quality, environmentally friendly and sustainable task. Yet SCC opposes it on the foundation that it includes a minor flood defence element to which it objects. It does not seem to accept that Cork metropolis Council is obliged to have regard to flood risks when offering new facilities or public realm enhancements in low lying areas and to reply correctly to those dangers. And on and on it goes. SCC doesn’t desire the Morrison’s Island Public Realm assignment, the Docklands to metropolis Centre road network Scheme, or LLFRS. It most effective needs what it needs. I see little point in purporting to “Love the Lee” and permitting its waters to lap across the ankles of those that are attempting to make a dwelling. Cork metropolis centre will proceed to flood except positive defences are in area. When critical flooding occurs once more, because it actually will, it might be unique to peer even if these antagonistic to those schemes should be seen shoulder to shoulder with metropolis Council group of workers, residents and enterprise homeowners working all the way through the evening to keep buildings and organizations. Will they be there mopping up premises and what words of comfort will they present beleaguered merchants another time dropped at their knees through avertable flooding?.

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